Modified radioimmunoassay for peripheral plasma aldosterone
نویسندگان
چکیده
منابع مشابه
A simplified radioimmunoassay of plasma aldosterone.
The analytical evaluation is described of a [125I]-aldosterone radioimmunoassay method for measuring aldosterone in human plasma, which requires no chromatographic purification before quantification by radioimmunoassay but does require organic solvent extraction. Rabbit anti-aldosterone serum is used, generated against aldosterone-3-oxime coupled to thyroglobulin. The antibody demonstrates negl...
متن کاملQuantitation of plasma aldosterone by radioimmunoassay.
Aldosterone antibodies were produced by injecting sheep with the albumin conjugate of aldosterone. 18,21-dihemisuccinate. Conditions optimal for an accurate, reproducible assay with this antiserum were determined experimentally. Aldosterone is extracted from plasma and isolated by a single thin.layer chromatographic system before the immunoassay is performed. Normal plasma aldosterone in supine...
متن کاملA direct radioimmunoassay for aldosterone in unextracted serum and plasma.
We describe a novel radioimmunoassay procedure for the direct estimation of aldosterone in unextracted plasma and serum samples, in which interfering binding proteins are digested by Proteinase K (Tritirachium alkaline proteinase, EC 3.4.21.14), a powerful proteolytic enzyme. Heating at 75 degrees C for 15 min inactivates the enzyme before radioimmunoassay. Alternatively, EDTA may be used to in...
متن کاملImproved extraction of aldosterone from plasma and urine before radioimmunoassay.
Radioimmunoassay of urinary or plasma aldosterone requires an initial extraction of steroids before purification and analysis. This extraction characteristically must be performed on acid-hydrolyzed urine or plasma, both of which have been supplemented with tnitiated aldosterone, to assess the analytical recovery. After extraction, the organic phase must be washed sequentially with 0.1 mol/lite...
متن کاملNon-chromatographic radioimmunoassay procedure for urinary aldosterone.
We describe a sensitive, specific, and simple procedure for measuring aldosterone in human urine, which requires no chromatographic purification before quantification by radioimmunoassay but does include hydrolysis and extraction steps. Rabbit anti-aldosterone serum is sued, generated against aldosterone-18,21-dihemisuccinate coupled to human serum albumin. The antibody cross reacted little wit...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1975
ISSN: 0014-5793
DOI: 10.1016/0014-5793(75)81074-x